“There was a note when we first turned the script [in],” Baumbach said. “On page 111: ‘Does a Mattel executive have to be shot?’ At the time we were like, that should just be on the ad!…
A credit card PIN is required for ATM cash advances and some purchases in foreign countries. Read on for more.
What's a Credit Card PIN and How Do I Get One?
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We investigated and described the thoracic structures, jumping mechanism, and promesothoracic interlocking mechanism of the click beetle Campsosternus auratus (Drury) (Elateridae: Dendrometrinae). Two experiments were conducted to reveal the critical muscles and sclerites involved in the jumping mechanism. They showed that M2 and M4 are essential clicking-related muscles. The prosternal process, the prosternal rest of the mesoventrite, the mesoventral cavity, the base of the elytra, and the posterodorsal evagination of the pronotum are critical clicking-related sclerites. The destruction of any of these muscles and sclerites resulted in the loss of normal clicking and jumping ability. The mesonotum was identified as a highly specialized saddle-shaped biological spring that can store elastic energy and release it abruptly. During the jumping process of C. auratus, M2 contracts to establish and latch the clicking system, and M4 contracts to generate energy. The specialized thoracic biological springs (e.g., the prosternum and mesonotum) and elastic cuticles store and abruptly release the colossal energy, which explosively raises the beetle body in a few milliseconds. The specialized trigger muscle for the release of the clicking was not found; our study supports the theory that the triggering of the clicking is due to the building-up of tension (i.e., elastic energy) in the system.
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Butterflies possess impressive cognitive abilities, and investigations into the neural mechanisms underlying these abilities are increasingly being conducted. Exploring butterfly neurobiology may require the isolation of larval, pupal, and/or adult brains for further molecular and histological experiments. This procedure has been largely described in the fruit fly, but a detailed description of butterfly brain dissections is still lacking. Here, we provide a detailed written and video protocol for the removal of Bicyclus anynana adult, pupal, and larval brains. This species is gradually becoming a popular model because it uses a large set of sensory modalities, displays plastic and hormonally controlled courtship behaviour, and learns visual mate preference and olfactory preferences that can be passed on to its offspring. The extracted brain can be used for downstream analyses, such as immunostaining, DNA or RNA extraction, and the procedure can be easily adapted to other lepidopteran species and life stages.
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